6 to 25 ng of prokaryotic recombinant protein reaching a plateau in concentrations above 25 ng. SpectraMax iD5. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. The solution was incubated for 15 min and fluorescence was measured on a SpectraMax microplate reader (Molecular Devices). 0´ 104 6. 100 fmol 384-well (75 µL) 0. The OD 600 was plotted against time using Graphpad Prism 8. SpectraMax Glo Steady-Luc Reporter Assay Kit. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 2. The plate was incubated for 1 hr at room temperature with shaking. Microplate formats can vary from 6-1536 wells, providing the ability to read many samples in a short time. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers can measure absorbance, fluorescence and luminescence. 003 fmol 0. SpectraMax® iD5 Multi-Mode Microplate Reader (Molecular Devices cat. 40266 Email: [email protected] or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers begining with. , Sunnyvale, CA, USA) and. Chapter 1: Introduction The FilterMax™ F3 Multi-Mode Microplate Reader and the FilterMax™ F5 Multi-Mode Microplate Reader are ideal for a broad range of applications, including drug discovery, genomics, proteomics, and cell-based research. MS-3 Laser Bar Code Reader. Equal concentrations of protein were separated in Mini-PROTEAN® TGX™ Precast 4–20% Gels (Bio-Rad, Hercules, CA, USA) and transferred to nitrocellulose. Multi-Mode microplate readers offer flexibility and ease of use over a broad range of applications. Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. Cell viability was measured in percentages (%) as compared to untreated cells, which were assumed to be 100% viable. 2 Temperature and cooling rate control. F3/F5 Multi-Mode Microplate Readers. A powerful and reliable microplate reader is what you need! Get access to readers which will ensure an optimal readout. To read the absorbance, a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA 95134, USA) with a wavelength of 595 nm was used. Close Search. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument se Figure 1: Schematic of a typical workow of the semi solid cloning process. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. Open SoftMax® Pro software. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. time plots at 30, 60, and 90 min using MATLAB® R2017b (9. Molecular Devices, LLC Reading Modes: Endpoint, Kinetic, Linear Scan, Area Scan; Optical System: LEDs, Deuterium Lamp; Wavelength Range: 230 to 750 nm;. After further centrifugation in the same fashion, the supernatant was subjected to subsequent enzymatic reactions. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. ScanLater Western Blot Detection System. Absorbance detection in the UV and visible range (230 nm to 650 nm) Fluorescence intensity; top read and bottom read. F3/F5 Multi-Mode Microplate ReadersThe plate was incubated for 1 hr at room temperature with shaking. The mixture was then boiled for 10 min. Reaction was stopped by the addition of 100 μl Stop Solution and optical density of each well was determined using a FilterMax F5 Multi-Mode microplate reader and SoftMax Pro 6. searchPolymerization and polymerization kinetics were monitored in a Corning 96-well black flat bottom polystyrene microplate (Corning, Lowell, MA, USA) using a FilterMax F5 Multi-Mode Microplate Reader. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. A standard curve was created by diluting the ATP standards to a range between 2 × 10 −3 and 20 μM ATP. Luminescence was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. HEK293T cells were harvested 48 h post-transfection in Passive Lysis Buffer (Promega). FilterMax F5 Multi-Mode Microplate Reader. In addition, the FilterMax F5 Reader is capable of measuring. SpectraMax MiniMax 300 Imaging Cytometer for the SpectraMax i3x Multi-Mode Microplate Detection Platform KEY FEATURES • ombine cellular imaging C and microplate assays into a single workflow • liminate cell staining for cell E counting and confluency with StainFree Technology • omplete cell viability C and toxicity assays with. please click here. Internal controls were. 7°C (gradient heating design). All experiments were performed in triplicate. Select the protocol “Concentration_pico_green_v3. MD, MPH, Milton J. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe OD 405 was measured in a FilterMax™ F5 Multi-Mode Microplate Reader. Scan measurement types: endpoint, kinetic, multiple wavelength, linear scan and area scan. Figure 2: Images of colonies of commonly used cell types raised in semi-solid culture in CloneMedia. Citations: Dual monochromators increase performance and assay flexibility. They are very complex instruments. Affordability with unmatched assay performance in a multi-mode microplate reader The FilterMax™ F3 and F5 Multi-Mode Microplate Readers provide unmatched value and flexibility for a variety of applications capable of performing: - Absorbance detection in the visible range (340 nm to 650 nm, F3) or UV and visible range (230nm to. The FilterMax™ F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), and time-resolved fluorescence (TRF) for 6- to 1536. Molecular Devices Multi-Mode Microplate Reader FilterMax F5 $7,995. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Download Table of Contents. ”SpectraMax® Paradigm® Multi-Mode Detection Platform. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 00 USD. ScanLater Western Blot Detection System. 3. All ELISA kits were read using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices; San Jose, CA, USA), according to the kit guidelines. FilterMax F5 Multi-Mode Microplate Reader. Filters: Excitation and emission slides for 4 filters each. 5 mP. , San Diego, CA, USA). Relative firefly and Renilla luciferase units were measured in a FilterMax F5 Multi-Mode Microplate Reader using the Dual-Luciferase ® Reporter Assay System (Promega) according to the manufacturer’s instructions. Users can program endpoint, kinetic, multiple wavelength, linear scan and area scan measurement types. cruzi incubated for 1 h with sertraline at 2 μM. SpectraMax Quant dsDNA Assay Kits. Fluorescence intensity was determined using a FilterMax F5 multi-mode microplate reader at an excitation wavelength of 485 and an emission wavelength of 530nm. 8 Pages. 561. . Instrument Configuration: Assay Technology Adapta® & LanthaScreen® Europium& FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. The increase in NADH, an end product in this reaction, was determined by. FilterMax™ F3 and F5 Multimode Microplate Readers, Molecular Devices. FilterMax F3 & F5 Microplate Readers. BMG Labtech - FLUOSTAR OMEGA. In this work, we studied the activity of the antidepressant drug sertraline against Trypanosoma cruzi trypomastigotes and intracellular. By category. FilterMax F5 Multi-Mode Microplate Reader. In addition, the instrument features linear and orbital shaking, plus temperature. Consult Molecular Devices's entire F3/F5 Multi-Mode Microplate Readers catalogue on MedicalExpo. This assay measures cellular metabolic activity as an indicator of cell viability and proliferation. Samples were considered positive if the average sample absorbance was greater than twice the mean absorbance of healthy. Excitation Filter – EXP1 F535/25, F485/20, F485/20, F370/TRF. ** Some compatible filter sets are available for purchase from Life. 2 Pages. Datapaq Express (Vial Rack Bottom Label 2D Reader) XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING. 003 fmol 0. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. org) with optimized instrument settings b. After the absorbance was measured at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA), the concentrations of the samples were determined by. FilterMax F5 Multi-Mode Microplate Reader. 4 Pages. 00 USD. F3/F5 Multi-Mode Microplate Readers. 561. The FilterMax F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), time-resolved fluorescence (TRF), for 6- to 384-well plates. Molecular Devices Microplate reader VersaMax $1,495. CloneMatrix. , GFP expression yields from High Five cells infected with the two viruses were estimated using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). GenePix 4300/4400 Microarray Scanner. . An anti-human HER2/neu human IgG3/κ (Huang and Morrison, 2006) was used as isotype (negative) control. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. FilterMax F5 Multi-Mode Microplate Reader, Molecular Devices SpectraMax 340PC384 Microplate Reader, Molecular Devices. Each condition was performed in. Open the catalog to page 1. After incubation for 2 h at 37 °C, the mixture was centrifuged at 2200 rpm for 10 min to obtain the supernatant. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. The ELISA test was performed using the supernatants from the morphological test. Caspase-3 activity was used as a marker of cell apoptosis and was assessed according to. com B. Then, 50 µL of a stop solution were applied to terminate the reaction. Synergy™ H1 is a flexible monochromator-based multi-mode microplate reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Hit enter to search or ESC to close. com A. Renilla/Firefly luciferase activities were measured twice. Compare Fluorescence Microplate Readers from leading suppliers on Biocompare. F5 Multi-Mode Microplate Reader. Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader + + Molecular Devices FlexStation. 5 million in 2019 and is projected to reach US$ 704. Match case Limit results 1 per page. Page: 1/4NADPH turnover was measured on FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Francisco, USA) at 340 nm over 30 min at 37 °C. Absorbance microplate assays For more than 30 years, absorbance-based detection has been the preferred mode for many microplate-based assays, such as ELISA, protein, and nucleic acid quantitation or enzymatic assays. Table of Contents. Fold changes were calculated as the area under the curve of stimulated HKLs relative to unstimulated HKLs (RPMI without zymosan). Our newest multimode microplate reader combines simplicity with top-of-the-line performance. MetaMorph NX. Click to Contact Seller. The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. Molecular Devices Multi-Mode Microplate Reader FilterMax F5. 8. Multi-mode microplate readers are used in different research fields for the quantification of different biological and chemical assays in a microplate. A powerful and reliable microplate reader is what you need! Get access to readers which will ensure an optimal readout. Statistical analysis was performed using. MetaMorph NX. 40266 Email: drugdiscoverytech@lifetech. 2. • DNA/RNA quantitation • Reporter gene assay The FilterMax™ F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), and time-resolved fluorescence (TRF) for 6- to 1536-well plates. After 30 min, absorbance of the lysates at 405 nm was measured on a microplate reader (FilterMax F5 Multi-Mode microplate reader). Location: San Diego, California, UNITED STATES Member Since: 17th September, 2003 (20 years ago) Current Listings: 667 Shipping Policy: Freight Shipping and Handling: For all orders, Name/Company Name, Address, and Contact Phone Number are required. 3 Pages. Activation of TLR4 and TLR2 receptors. 4 Pages. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Related Products: FilterMax F3 & F5 Multi-Mode Microplate Readers, SpectraMax i3 Multi-Mode Platform Microplate Detection System,. #6590-0121)The MTT solution was withdrawn, and 200 µl of dimethyl sulphoxide (DMSO) was added to determine the absorbance values, at an absorption curve of λ= 595 nm (FilterMax F5 Multi-Mode Microplate reader, Molecular Probes). A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. Maximum haemolysis was obtained using ultrapure distilled water, and untreated erythrocytes were used as a negative control [14]. The absorbance was measured at 450 nm with a FilterMax F3 & F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). F3 F5 F3-SC F5-SC F3 F5. • H1_M_FI_T_MUB. Description Z-height focusing is enabled for fast top- and bottom-reads for all read modes. Both the FilterMax F3 and F5 Readers are capable of detecting nucleic acid concentration by fluorogenic means. The amount of colorimetric product was continuously monitored for 120 min. . BIO TEK Synergy? 2 Multi-Detection Microplate Reader with Luminescence and Dual Reagent Dispenser SPECTRA MAX 190 MICROPLATE READER (90V TO 240V) WITH CURRENT BMG FLUOSTAR OPTIMA PLATE READER Molecular Devices Filtermax F5 Multi-mode Microplate Reader BioLogic VMP3 Potentiostat Chassis w/9 VMP3-01 Boards potentiostat FilterMax F5 Multi-Mode Microplate Reader. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 2 mm) was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 15 min over 16 h incubation time. Plate Formats:. Seoul, South Korea. 4. 2. Cells were seeded in 24-well plates and cultured. Unsuccessful migration. [The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. HEK293T cells were harvested 48 h post-transfection in Passive Lysis Buffer (Promega). Time-resolved Fluorescence. The results are expressed as cell viabilities (%) based on the control condition. 2 μM ATP standard was. Other recommended products. Depending on the model, Cytation 5 detection modes include fluorescence intensity (FI), fluorescence polarization (FP), time-resolved fluorescence (TRF), luminescence, UV-visible absorbance, imaging, and alpha. In . When recombinant supernatants were tested for the presence of Il10, only values within the linear range of. Instrument Configuration: Assay Technology Adapta® & FilterMax F5 Multi-Mode Microplate Reader. 3 Pages. Load prepared PicoGreen plate (step C. 3 software (Molecular Devices). Click Run. Topic. Capabilities: FilterMax F5 Multi-mode microplate reader. ScanLater Western Blot Detection System. #F5) Methods Standard curves were generated following the assay protocols provided with each kit. Select preinstalled protocol on the GloMax® Instrument and pre-warm instrument. 100 fmol 384-well (75 µL) 0. SpectraMax Quant dsDNA Assay Kits. Please note it is NOT a Keyspan USA-19 HS USB adaptor that is commonly used by. 3 Pages. Imaging by immunofluorescence. Neurotransmitter Transporter Uptake Assay Kit. The amount of colorimetric product was continuously monitored for 120 min. 9. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . At least three independent experiments were performed for each. Plates were read at absorbance 405 nm using a FilterMax F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). 561. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry. 3D cell proliferation assays were conducted using high concentrations to optimize conditions for cell lines in 20, 100 or• FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Manufacturer: Molecular Devices Model: FilterMax F5 Condition: Used. Related Instruments Agilent BioTek BioStack microplate stacker Automate routine microplate washing or dispensing processes with the compact BioStack microplate stacker. Thermo Fisher Scientific. A microplate reader is laboratory machine used for the estimation of biochemical or. FilterMax F5 Multi-Mode Microplate Reader. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. Molecular Devices’ FilterMaxTM F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. 100 fmol 1536-well (8 µL) 0. The drawing shows a standard microplate of 15 mm height covered with a lid. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumFlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Key Features • Five-mode reader with wide range of applications • Flexible liquid transfer enables more assay conditions • User-defined pipetting simplifies assay optimization • Instrument and software validation Adapting to biochemical- and cell. 3 Pages. FilterMax F3 / FilterMax F5: Inquire: SpectraMax iD3 and iD5: SpectraMax M: Price: Description: Unlimited breadth of user-upgradeable application modules expands research capabilities. , Sunnyvale, CA, USA). Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 3 Pages. MetaFluor Software. book Page 1 Tuesday, September 7, 2010 6:14 PM. Fluorescence polarization was measured at 21°C using FilterMax™ F5 Multimode Microplate Reader (Molecular Devices) with excitation and emission wavelengths of 485 nm and 535 nm, respectively. 05); b Evaluation of the ATP levels of T. SpectraMax Paradigm Multi-Mode Safety Guide; SpectraMax Paradigm Multi-Mode Unpacking Guide; SpectraMax Paradigm Multi-Mode. 00. 5. ** Some compatible filter sets are available for purchase from Life. 3 Pages. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. Briefly, serial dilutions of TNP were incubated with 200 TCID 50 virus in presence of diethylaminoethyl-dextran and the neutralizing activity was assessed by measuring luciferase activity after 48 h using a FilterMax F5 multi-mode microplate reader (Molecular Devices). CloneMatrix. 2 Pages. The medium was then removed and the cells washed once with 200 μl of ice-cold phosphate buffered saline (PBS). 00 USD. Plate stacker and robot integration The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. There is no programming of protocols or wet chemistry involved –just select the mode(s) you want to test, insert the plate and go. Click on the microplate icon in the upper left corner and then click "New" to open a new data file: In the section "Plate1", click on the Settings icon. In addition, the FilterMax F5 Multi-Mode Microplate Do you want to learn how to use the FilterMax F3 and F5 Multi-Mode Microplate Readers for your research? Download the user guide and find out how to install, operate, and troubleshoot the instruments. The experiment was carried out with four biological replicates and two technical replicates. 3 and higher include optimized reader settings, as well as data analysis, for faster results. prt Read the plate and then save the experiment. Add. 5 ml 0. Add to my manuals. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® Europium• FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. 4. Microplate Readers. Other technologies such as imaging. MICROPLATE READERS Multi-Mode Readers. Neurotransmitter Transporter Uptake Assay Kit. 012 fmol 0. FilterMax F5 Multi-mode microplate reader. , Sunnyvale, CA, USA) for 30 min and 1 h after dye addition. 4 Pages. , call 1-800-635-5577. A dual-color pyrometer with a selected wavelength away from that of the laser radiation has been used to monitor the melt pool temperature. The instrument performs an initialization procedure that moves the optics and the microplate drawer to home positions. 380 or. The donor and acceptor can be used to label a variety of biomolecules, for. 1 at 600 nm as determined using a FilterMax F5 multi-mode microplate reader (Molecular Devices). The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell. Run a variety of luminometric research applications with the Thermo Scientific Luminoskan microplate luminometer, a compact and robust instrument offering versatile plate formats, fast reading speeds and up to three dispensers. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seF3/F5 Multi-Mode Microplate Readers. i3x, SpectraMax M4 or M5, and FilterMax F5 Multi-Mode Microplate Readers. 2 Pages. The experiment was carried out with two biological replicates and three technical replicates. To do so, perform one of the. 713579). Add to cart. 1% (v/v) formic acid, was adopted for lipid separation: 0–2 min. F3/F5 Multi-Mode Microplate Readers The FilterMax Series of microplate readers includes: FilterMax F3 Multi-Mode Microplate Reader. In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. Ammonia and NO 2 Ϫ / NO 3 Ϫ analyses were conducted according to established protocols (37, 38), using a FilterMax F5 multimode microplate reader (Molecular Devices, Canada). Each condition was performed in. Compact designs make these instruments. : 28 Sept 12 Page 2 of 20 Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. #0200-7030) Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. VisionMate 96 (Vial Rack Bottom Label 2D Reader) Ziath. 2. Multi-mode Microplate Readers. SpectraMax M-series Microplate readers. SoftMax Pro Software, data can be visualized as gray scale, kinetic plots or reaction rates. 3 Pages. , Sunnyvale, CA, USA) and normalized to the. : F5: Accuracy: \ Detection Limit (384-Well): 0. The supernatant was taken and 0. For FcγRI (high affinity receptor) binding assays, plates were coated with 1 μg/mL recombinant human soluble. Sunnyvale, Calif. Add to cart. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumThe parasite viability was determined by resazurin assay (0. This instrument has passed specific verification criteria. Labsystems Fluoroskan Ascent FL Microplate Fluorometer $3,250. FilterMax® F3 & F5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell viability, ELISA, Fluorescence,. The inter-assay coefficient of variations (CV) and values for all biomarkers can be found in Table 4. Luminescence (glow) Fluorescence polarization. It can be upgraded into a powerful multi-mode reader with up to seven detection modes. performs within its specifications. This document is provided to customers. The fluorescence was determined using a fluorimetric microplate reader (FilterMax F5 Multi-Mode Microplate Reader) at 540 and 595 nm for excitation and emission, respectively. RNA extraction and reverse transcriptase–quantitative PCR. After incubation for 2 h at 37 °C, the mixture was centrifuged at 2200 rpm for 10 min to obtain the supernatant. Cells were co-transfected with 2 μg total plasmid DNA (1760 ng empty vector or proviral plasmid DNA. The other members of the E39 family (525i, 530i, and 540i) do not have limited slip. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $7,900. Homogeneous Time-Resolved Fluorescence (HTRF) . ; Page 2 For research use only. 7. Plate Formats:. 3. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Click “Eject Plate Carrier. SpectraMax i3x Multi-Mode Detection Platform Biotek Synergy H1 Operator's Manual Appliskan User Manual FilterMax F3/F5 Multi- Mode Microplate Readers Spectramax® m2/m2 user guide User manual Software version 3. The standard curve presented a linear phase from 1. Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 5 μg/ml) in the dark for 1 h. BMG Labtech - THERMOstar. RLUs were determined using a FilterMax F5 multi-mode microplate reader (Beckman Coulter). The results were calculated using a malondialdehyde (MDA) curve and expressed as nmol MDA/mg protein. Affordability with unmatched assay performance in a multi-mode microplate reader The FilterMax™ F3 and F5 Multi-Mode Microplate Readers provide unmatched. After 30 min, absorbance of the lysates at 405 nm was measured on a microplate reader (FilterMax F5 Multi-Mode microplate reader). After further centrifugation in the same fashion, the supernatant was subjected to subsequent enzymatic reactions. Protein contamination can be measured at 280 nm. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. The. ABS Test results. 00. The following document is intended to demonstrate setup of this instrument. ScanLater Western Blot Detection System. ** Some compatible filter sets are available for. CloneMatrix. shopping cart 0 items $0. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. 100 fmol 1536-well (8 µL) 0. Time-resolved Fluorescence. 8”. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The absorbance was measured at 450 nm using a FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. The FlexStation 3 Benchtop Multi-Mode Microplate Reader can measure absorbance, fluorescence intensity, fluorescence polarization, luminescence and time-resolved fluorescence. Page 4 Operator’s Manual Intended Use Statement • The Synergy H1 is a hybrid multi-mode microplate reader. Once the FilterMax F5 has completely powered on, double-click on the Multi- Mode Analysis Software. 3 Pages. Not for use in diagnostic procedures. In addition, the instrument features linear and orbital shaking, plus temperature control. 3 Pages. : 28 Sept 12 Page 2 of 20 Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Page 38 | Getting Started Overview The Cytation 5 is a hybrid multi-mode microplate reader. 4 Pages. The absorbance was read with a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA, USA) at a wavelength of 595 nm. The experiment was performed in 4 technical replicates (wells in the cell culture plate). CloneMatrix. FilterMax F3 & F5 Microplate Readers. SpectraMax Glo Steady-Luc Reporter Assay Kit. SpectraMax Quant dsDNA Assay Kits. (PMT) operates in photon counting mode, affording increased sensitivity for red dyes and a linear dynamic range over 6 orders of magnitude. the luminescence reading was taken on a FilterMax F5 Multi-Mode. Accurate measurements are ensured by automatic lamp calibration prior to each reading. Renilla/Firefly luciferase activities were measured twice.